目的:探讨定量检测骨桥蛋白编码基因在原发性肺癌中的表达及其意义。方法: 制备24例肺癌患者癌及癌周正常肺组织中总RNA，采用RT-PCR及实时荧光定量PCR技术分析spp1基因的mRNA表达水平。结果:在所有24例肺癌中肺癌组织的骨桥蛋白表达高于相邻的癌周正常肺组织，并且全部在6倍以上，其中7例在100倍以上。结论: 肺癌组织中骨桥蛋白的表达明显升高，是肺癌诊断的重要标志物。

Objective: To quantitatively detect the expression level of spp1 gene in lung carcinoma. Method: The RT-PCR and real-time fluorescence quantitative RT-PCR method was used. Result: The spp1 gene expressed higher in tumor tissue than that in normal tissue in all 24 cases of lung carcinoma. In 9 cases spp1 expression level was about or more than 100 times as that of normal lung tissue. Conclusion: The spp1 gene expressed higher in lung carcinoma tissue than that of normal lung tissue and indicated that it might be an important marker in the diagnosis of the lung cancer.

国家自然科学基金面上项目（30270686）资助