目的:探讨过表达调节因子X1 基因（regulatory factor X1，RFX1）对神经胶质瘤细胞株F98 细胞增殖和侵袭能力的影响及其作用机制。方法：用慢病毒转染法将RFX1 转染到F98 细胞，构建过表达RFX1 的F98 细胞株（F98-RFX1 组），同时设立空质粒对照组（F98-Vector 组）和正常培养组（F98 组）。用计数法观察过表达RFX1 对各组F98 细胞增殖的影响，用AnnexinV/PI 染色法流式细胞术检测过表达RFX1 对F98 细胞凋亡的影响，用Transwell 小室法检测过表达RFX1 对F98 细胞侵袭能力的影响。结果：成功建立过表达RFX1 胶质瘤F98 细胞株。过表达RFX1 组胶质瘤F98 细胞的增殖能力显著低于F98 组[48 h: （12.08±2.17）×104/ml vs（23.67±4.51）×104/ml，P<0.05]和F98-Vector 组[96 h: （8.17±0.31）×104/ml vs（18.58±1.18）×104/ml；P<0.05]；过表达RFX1 组细胞的凋亡水平明显上升[ （21.89±2.33）% vs（3.38±1.39）%、（10.42±1.83）%，P<0.05]；过表达RFX1 组细胞侵袭能力显著下降[ （33.3±7.99）vs（56.5±13.9）、（60.6±11.8）个，P<0.01]。结论：RFX1 可以调控增殖和侵袭相关基因的表达，进而抑制胶质瘤细胞的增殖和侵袭能力、促进细胞凋亡。

Objective: To validate the effect and the possible mechanism of human regulatory factor X1 (RFX1) over-expression on the proliferation and invasion of glioma F98 cells. Methods: RFX1-overexpressed F98 cells (F98-RFX1 group) were constructed by lentivirus transfection, a control group (F98-Vector group) and normal group (F98 group) were established. The effect of RFX1 over-expression on F98 cell proliferation was observed with counting method, cell apoptosis was determined by AnnexinV-PI staining, and the cell invasion was observed with Transwell method. Results: F98 cell line over-expressing RFX1 was successfully established. The proliferation capacity of F98-RFX1 group was significantly lower than that of F98 group (48 h: [12.08±2.17]×104/ml vs [23.67±4.51]×104/ml, P<0.05] and F98-Vector group (96 h: [8.17±0.31]×104 /ml vs [18.58±1.18]×104 /ml, P<0.05); The apoptosis level of cells in F98-RFX1 group was significantly increased ([21.89±2.33]% vs [3.38±1.39]%, [10.42±1.83]%, P<0.05]; The invasiveness of cells in F98-RFX1 group was significantly reduced ([33.3±7.99] vs [56.5±13.9], [60.6±11.8], P<0.01). Conclusion: RFX1 can regulate the expression of genes related with proliferation and invasion, thereby inhibiting the proliferation and invasion ability of glioma cells and promote cell apoptosis.

山西医科大学汾阳学院科技发展基金资助项目（No.2016B05)