目的：探讨 lncRNA SNHG14 对甲状腺癌 SW579细胞恶性生物学行为的影响及其分子机制。方法：收集2017年10月至2018年12月青海省人民医院收治的20例甲状腺癌患者的癌组织及癌旁组织标本，用qPCR检测甲状腺癌组织和对应癌旁组织中SNHG14与miR-433-3p 的表达；根据转染物的不同，将 SW579 细胞分为 si-NC 组（转染si-NC）、si-SNHG14组（转染si-SNHG14）、miR-NC组（转染 miR-NC）、miR-433-3p mimic 组（转染 miR-433-3p mimic）、si-SNHG14+anti-miR-NC 组（共转染si-SNHG14与anti-miR-NC）和si-SNHG14+anti-miR-433-3p组（共转染si-SNHG14与anti-miR-433-3p）。MTT法、FCM、Transwell 实验分别检测转染后SW579细胞的增殖能力、细胞周期、细胞凋亡率、迁移及侵袭能力的改变；利用双荧光素酶报告基因实验检测SNHG14是否可结合miR-433-3p，qPCR法检测SNHG14与miR-433-3p之间的相互调控关系。结果：SNHG14在甲状腺癌组织中的表达高于癌旁组织（P<0.05），而miR-433-3p的表达水平低于癌旁组织（P<0.05）。抑制SNHG14的表达或过表达miR-433-3p 可使SW579细胞增殖能力降低（P<0.05）、迁移与侵袭细胞数减少（均 P<0.05）、细胞凋亡率升高（P<0.05）、G1期细胞比例升高（P<0.05）且S期细胞比例降低（P<0.05）。双荧光素酶报告基因实验证明SNHG14可结合miR-433-3p，抑制SNHG14的表达可提高SW579细胞中miR-433-3p水平（均P<0.05）。同时抑制miR-433-3p 和SNHG14的表达可部分逆转后者对SW579细胞的增殖、凋亡、迁移和侵袭的作用（均P<0.05）。结论：甲状腺癌组织中lncRNA SNHG14呈高表达、miR-433-3p呈低表达，lncRNA SNHG14可通过靶向结合miR-433-3p促进甲状腺癌SW579细胞的增殖、迁移、侵袭而抑制细胞凋亡。

Objective: To explore the effect of lncRNA SNHG14 on the malignant biological behaviors of thyroid cancer SW579 cells and its mechanism. Methods: The cancer tissue and paracancerous tissue samples of 20 patients with thyroid cancer admitted to Qinghai Provincial People's Hospital from October 2017 to December 2018 were collected. The expression of SNHG14 and miR-433-3p in thyroid carcinoma tissues and corresponding paracancerous tissues was determined by qPCR. Depending on the transfectant, SW579 cells were divided into the si-NC group (transfected with si-NC), si-SNHG14 group (transfected with si-SNHG14), miR-NC group (transfected with miR-NC), and miR-433-3p mimic group (transfected with miR-433-3p mimic), si-SNHG14+anti-miR-NC group (co-transfected with si-SNHG14 and anti-miR-NC), and si-SNHG14+anti-miR-433-3p group (co-transfected with si-SNHG14 and anti-miR-433-3p). MTT method, FCM, and Transwell test were used to detect the changes in proliferation, cell cycle, apoptosis rate, migration, and invasion ability of SW579 cells after transfection, respectively. The dual-luciferase reporter gene assay was employed to analyze whether SNHG14 could bind to miR-433- 3p, and the regulation relationship between SNHG14 and miR-433-3p was detected by qPCR. Results: Compared with the paracancerous tissues, the expression of SNHG14 in thyroid carcinoma tissues was significantly increased while the expression level of miR-433-3p was obviously decreased (all PObjective: To explore the effect of lncRNA SNHG14 on the malignant biological behaviors of thyroid cancer SW579 cells and its mechanism. Methods: The cancer tissue and paracancerous tissue samples of 20 patients with thyroid cancer admitted to Qinghai Provincial People's Hospital from October 2017 to December 2018 were collected. The expression of SNHG14 and miR-433-3p in thyroid carcinoma tissues and corresponding paracancerous tissues was determined by qPCR. Depending on the transfectant, SW579 cells were divided into the si-NC group (transfected with si-NC), si-SNHG14 group (transfected with si-SNHG14), miR-NC group (transfected with miR-NC),and miR-433-3p mimic group (transfected with miR-433-3p mimic), si-SNHG14+anti-miR-NC group (co-transfected with si-SNHG14 and anti-miR-NC), and si-SNHG14+anti-miR-433-3p group (co-transfected with si-SNHG14 and anti-miR-433-3p). MTT method, FCM,and Transwell test were used to detect the changes in proliferation, cell cycle, apoptosis rate, migration, and invasion ability of SW579 cells after transfection, respectively. The dual-luciferase reporter gene assay was employed to analyze whether SNHG14 could bind to miR-433-3p, and the regulation relationship between SNHG14 and miR-433-3p was detected by qPCR. Results: Compared with the paracancerous tissues, the expression of SNHG14 in thyroid carcinoma tissues was significantly increased while the expression level of miR-433-3p was obviously decreased (all P<0.05). SNHG14 inhibition or miR-433-3p overexpression could reduce cell proliferation, the number of migrated and invaded cells (P<0.05), increase the rate of apoptosis (P<0.05) and the proportion of cells in the G1 phase (P<0.05), and decrease the proportion of cells in the S phase (P<0.05). Dual-luciferase reporter gene assay demonstrated that SNHG14 could bind to miR-433-3p, and inhibition of SNHG14 expression could promote the expression of miR-433-3p in SW579 cells (all P<0.05). Simultaneous inhibition of miR-433-3p expression partialy reversed the inhibitory effect of SNHG14 downregulation on cell proliferation, apoptosis, migration, and invasion of SW579 cells (all P<0.05). Conclusion: lncRNA SNHG14 is highly expressed while miR-433-3p is lowly expressed in thyroid cancer tissues. lncRNA SNHG14 can promote the proliferation, migration and invasion and inhibit apoptosis of thyroid cancer SW579 cells by targeted binding to miR-433-3p.

2020年青海省卫生健康科研课题资助项目（No.2020-wjzdx-42）