[摘 要] 目的：探讨溶酶体相关膜蛋白3（LAMP3）与转录激活因子4（ATF4）在宫颈癌中的表达及其与临床病理参数的相关 性。方法：采用免疫组化SP法检测正常宫颈组织、宫颈上皮内病变组织及宫颈癌组织中LAMP3与ATF4的表达情况，分析两种 蛋白与宫颈癌患者临床病理参数之间的关系，并且分析两种蛋白的相关性。结果：LAMP3在正常宫颈组及高级别鳞状上皮内 病变组中均为阴性或低表达，在宫颈癌中高表达率为38.3%，差异有统计学意义（χ2 = 14.113，P = 0.001）。ATF4在正常宫颈组中 高表达率为26.7%，在高级别鳞状上皮内病变组中高表达率为10.0%，在宫颈癌组中高表达率为58.3%，差异有统计学意义（χ2 = 11.078，P = 0.004）。LAMP3的表达与宫颈癌FIGO分期（χ2 = 10.139，P = 0.006）、淋巴结转移（χ2 = 8.475，P = 0.004）有关，差异均 有统计学意义。ATF4的表达在宫颈癌病灶大小（χ2 = 4.578，P = 0.032）、FIGO分期（χ2 = 8.971，P = 0.009）、淋巴结转移（χ2 = 7.881， P = 0.005）等方面的差异均有统计学意义。LAMP3与ATF4在宫颈癌中的表达呈正相关（r = 0.388，P = 0.002）。结论：LAMP3与 ATF4在宫颈癌组织中表达升高，两者的表达程度具有相关性，且在宫颈癌的发生发展中发挥重要的促进作用，有望成为宫颈癌 治疗的潜在靶点。

[Abstract] Objective: To investigate the expression of lysosome-associated membrane protein 3 (LAMP3) and activating transcription factor 4 (ATF4) in cervical cancer and their correlation with clinicopathological parameters. Methods: The expression of LAMP3 and ATF4 in normal cervical tissues, cervical intraepithelial lesions, and cervical cancer tissues was detected by SP immunohistochemistry. The relationships between the expression of these two proteins and patient clinicopathological parameters were analyzed, as well as the correlation between LAMP3 and ATF4 expression. Results: LAMP3 was negative or lowly expressed in both normal cervix tissues and high-grade squamous intraepithelial lesions, but highly expressed in cervical cancer tissues (38.3%), showing a statistically significant difference (χ2 = 14.113, P = 0.001). ATF4 was highly expressed in 26.7% of the normal cervix tissues, 10.0% of the high-grade squamous intraepithelial lesions, and 58.3% in the cervical cancer tissues, also showing a significant difference (χ2 = 11.078, P = 0.004). In cervical cancer, LAMP3 expression was significantly associated with FIGO stage (χ2 = 10.139, P = 0.006) and lymph node metastasis (χ2 = 8.475, P = 0.004); ATF4 expression was significantly associated with tumor size (χ2 = 4.578, P = 0.032), FIGO stage (χ2 = 8.971, P = 0.009), and lymph node metastasis (χ2 = 7.881, P = 0.005). There was a positive correlation between the expression of LAMP3 and ATF4 in cervical cancer tissues (r = 0.388, P = 0.002). Conclusion: LAMP3 and ATF4 are highly expressed in cervical cancer tissues and are positively correlated. Both of them play important roles in the development and progression of cervical cancer and could serve as potential therapeutic targets for cervical cancer treatment.

青岛市医药卫生科研指导项目（No.2022-WJZD188）