[摘 要] 目的：探究环状RNA hsa_circ_0081621对人喉鳞状细胞癌AMC-HN-8和TU177细胞恶性生物学行为的影响。 方法：常规培养AMC-HN-8和TU177细胞，用转染试剂将si-NC、si-hsa_circ_0081621、空载体（vector）和hsa_circ_0081621过表达 载体（hsa_circ_0081621-OE）转染至AMC-HN-8和TU177细胞，分别记作si-NC、si-hsa_circ_0081621、vector和hsa_circ_0081621-OE 组。用CCK-8法、克隆形成实验、划痕愈合实验和Transwell小室实验分别检测敲减或过表达hsa_circ_0081621对AMC-HN-8和 TU177细胞增殖、迁移和侵袭能力的影响。结果：在AMC-HN-8和TU177中成功地敲减或过表达了hsa_circ_0081621；敲减或过 表达hsa_circ_0081621 能显著抑制或促进AMC-HN-8和TU177细胞的增殖、迁移和侵袭能力（P < 0.01或P < 0.001或P < 0.000 1）。 结论：hsa_circ_0081621可促进人喉鳞状细胞癌AMC-HN-8和TU177细胞的恶性生物学行为。

[Abstract] Objective: To investigate the effects of circular RNA (circRNA) hsa_circ_0081621 on the malignant biological behaviors of human laryngeal squamous cell carcinoma AMC-HN-8 and TU177 cells. Methods: AMC-HN-8 and TU177 cells were routinely cultured. si-NC, si-hsa_circ_0081621, empty vector (vector), and hsa_circ_0081621 overexpression vector (hsa_circ_0081621-OE) were transfected into AMC-HN-8 and TU177 cells, namely si-NC, si-hsa_circ_0081621, vector, and hsa_circ_0081621-OE groups, respectively. The effects of knockdown or overexpression of hsa_circ_0081621 on the proliferation, migration, and invasion of AMC-HN-8 and TU177 cells were detected by CCK-8 assay, colony formation assay, scratch wound healing assay, and Transwell chamber assay. Results: Successful knockdown or overexpression of hsa_circ_0081621 was achieved in AMC-HN-8 and TU177 cells. hsa_circ_0081621 knockdown significantly inhibited while hsa_circ_0081621 overexpression significantly promoted the proliferation, migration, and invasion of AMC-HN-8 and TU177 cells (P < 0.01 or P < 0.001 or P < 0.0001). Conclusion: hsa_circ_0081621 promotes the malignant biological behaviors of human laryngeal squamous cell carcinoma AMC-HN-8 and TU177 cells.

河北省省级科技计划 [No.（2020）20377709D]