[摘 要] 目的：通过构建小鼠ID8细胞腹水移植瘤模型，评估不同肿瘤负荷下的免疫细胞组成及细胞因子网络特征，为卵巢癌免疫微环境研究提供实验依据。方法：选用6~8周龄雌性C57BL/6小鼠，分为未注射ID8细胞的对照组和ID8细胞组，根据注射细胞数量将ID8细胞组分为1 × 10?组、5 × 10?组、1 × 10?组。用多细胞因子检测技术检测血清和腹水中 IFN-γ、IL-2、IL-6、TNF-α、CCL2、CXCL10、IL-17、IL-1β的浓度，流式细胞术检测腹水中 CD3? T细胞、CD4? T细胞、CD8? T细胞、CD19? B细胞、F4/80?CD11b?巨噬细胞、髓源性抑制细胞（MDSC）、调节性T细胞（Treg细胞）和NK细胞的比例。结果：ID8细胞腹腔移植后，ID8细胞组小鼠体质量和腹围均明显增加（均P < 0.05）。ID8细胞组血清中IL-6、IL-2、TNF-α、IL-1β水平显著升高（均P < 0.05），CCL2水平显著降低（P < 0.05），IFN-γ、IL-17、CXCL10无显著变化；与 ID8细胞组血清比较，ID8细胞组腹水中IL-6、IL-2、TNF-α、CCL2、CXCL10、IL-17水平均显著升高（均P < 0.05），IL-1β和IFN-γ含量均无显著变化。ID8细胞组腹水中的免疫细胞比例分析结果显示，CD19?B细胞占比最高，其次为F4/80?CD11b?巨噬细胞、CD3? T细胞，NK细胞比例最低。结论：ID8细胞腹水移植瘤模型可有效模拟卵巢癌局部免疫激活与全身炎症反应的失衡状态，肿瘤负荷通过调控T细胞、巨噬细胞等免疫细胞及血清-腹水差异化的细胞因子网络影响免疫景观，为卵巢癌免疫治疗提供了新靶点。

[Abstract] Objective: To establish a mice ascitic tumor model using ID8 cells and evaluate the immune cell composition and cytokine network features under different tumor burdens, providing experimental evidence for the study of the ovarian cancer immune microenvironment. Methods: Female C57BL/6 mice aged 6-8 weeks were selected and divided into two groups: a control group (without ID8 cell injection) and an ID8 cell group. The ID8 cell group was further subdivided into three subgroups based on the number of injected cells: 1 × 10? group, 5 × 10? group, and 1 × 10? group. Multiplex cytokine detection technology was used to measure the concentrations of IFN-γ, IL-2, IL-6, TNF-α, CCL2, CXCL10, IL-17, and IL-1β in the serum and ascites. Flow cytometry was employed to determine the proportions of CD3? T cells, CD4? T cells, CD8? T cells, CD19? B cells, F4/80?CD11b? macrophages, myeloid-derived suppressor cells (MDSC), regulatory T cells (Treg cells), and natural killer (NK) cells in the ascites. Results: After intraperitoneal transplantation of ID8 cells, the body weight and abdominal circumference of mice in the ID8 cell group were significantly increased (all P < 0.05). In the ID8 cell group, the serum levels of IL-6, IL-2, TNF-α, and IL-1β were significantly elevated (all P < 0.05), while the serum level of CCL2 was significantly decreased (P < 0.05); no significant changes were observed in the serum levels of IFN-γ, IL-17, and CXCL10. Compared with the serum cytokine levels in the ID8 cell group, the ascites levels of IL-6, IL-2, TNF-α, CCL2, CXCL10, and IL-17 were significantly higher (all P < 0.05), whereas the ascites levels of IL-1β and IFN-γ showed no significant changes. Analysis of immune cell proportions in the ascites of the ID8 cell group revealed that CD19? B cells accounted for the highest proportion, followed by F4/80? CD11b? macrophages and CD3? T cells, while NK cells accounted for the lowest proportion. Conclusion：：The ID8 cell ascitic tumor model can effectively simulate the imbalance between local immune activation and systemic inflammatory response in ovarian cancer. Tumor burden modulates immune landscape by regulating immune cells such as T cells and macrophages, as well as the differential cytokine networks between serum and ascites, providing new targets for ovarian cancer immunotherapy.

[基金项目] 国家自然科学基金（No. 82373283）；河北省中医药管理局科研计划项目（No. T2025060）；天津市卫生健康委员会中医中西医结合科研课题（No. 2023204）