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Purification of Angiostatm and Its Inhibitory Effects on B16 Melanoma
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Abstract:
Objective: To investigate the efficiency of treatment with angiostatin on melanoma. Methods: Angiostatin was purified through affinity chromatography from human plasminogen fragments obtained by digesting human plasminogen with elastase. Results: The result of experiment in vitro showed that the purified angiostatin could significantly inhibit the proliferation of bovine aortic endothelial cells stimulated by bFGF. Systematic administration of angiostatin at does of 0.6 mg/kg d for 14 days, the tumor weight reduced about 77 % ( P < 0.05) . We established the melanoma metastasis model by injection of B16 cells through mouse tail vein. Under the same treatment for 18 days, the melanoma lung metastasis reduced about 65 % ( P < 0.05) . Conclusion: Our results supply the experimental evidence for the application of angiostatin in melanoma therapy.
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