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Variation of Bystander Effect of E coli. CD/HSV1-tk and Prodrugs System in vitro
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Abstract:
Objective: To investigate the variation in vitro bystander effect among human lung cancer cell lines aller transfection of pCEAcd-tk. Methods: pCEAcd-tk was extracted and transfected to six human lung cancer cell lines. Their sensitivity to 5- fluorocytosine (5-FC) and ganciclovir (GCV) was measured by MTT assay. Their bystander effect was also detendned. Re- sults: 1. The efficiency of lipid mediated gene transfer was very low; 2. Two out of six human lung cancer cell lines(SPCA-1, A549) have a high sensitivity to 5-FC and GCV; 3. Bystander effect: Two out of six human cancer cell lines(SPCA-1,SC) have a good bystander effect, and one out of them(A549) has a intermediate bystander effect, but three out of them (GM-82, HIAMP and L-78)have a poor bystander effect. Conclusion: There are a variation in vitro bystander effect in six human lung cancer cell lines after transfected with pCEAcd-tk.
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Clc Number:
R734.205 R730.51
