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Effects of E1A Gene on Chemosensitivity of Human Lung Adenocarcinoma Cells
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Abstract:
To investigate the effects of adenovirus type 5 E1A gene (Ad5E1A) on chemosensitivity of human lung adenocarcinoma cells. Methods: The recombinant pcDNA3-E1A, a mammalian-expressing vector was introduced into the Anip973 cells with lipofectAMINE. The cells resistant G418 were selected. Dishes (24-well) were inoculated with Anip973, Anip973-vect and Anip973-E1A cells. Subconfluent monolayers were treated with cisplatin, paclitaxel and VP16 for 24 h, respectively. Viability of the cells was quantitated with MTT method. Results: The sensitivity of Anip973-E1A cells to cytotoxic agents (cisplatin and paclitaxel) was dramatically increased. The IC 50 values for cisplatin and paclitaxel were reduced to approximate 7-fold in Anip973-E1A cells as compared with Anip973-vect cells. For VP16, the sensitivity was not changed. Conclusion: The results demonstrated that E1A gene was capable of enhancing the sensitivity of human lung adenocarcinoma cells to cytotoxic agents (cisplatin and paclitaxel), and the increased sensitivity was associated with the reduction of p185 level, suggesting the possibility of using E1A gene combined with anticancer agents to improve tumor treatment.
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