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Effect of Inhibiting the Expression of the First Subtype of the Monocarboxylate Transporter(MCT1) Gene on Proliferation and Apoptosis in Human Lung Adenocarcinoma Cells
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Abstract:
Objective: To study the effect of inhibiting the expreesion of the first subtype of the monocarboxylate transporter(MCT1) gene on proliferation and apoptosis in tumor cells.Methods:MCT1 antisense gene expression vector was introduced into A549 cells with electroporation to inhibit the expression of MCT1 gene. The positive colonies were selected by G418. The integration of MCT1 antisense gene and A549 genomic DNA was confirmed by PCR and RT-PCR technique. The pHi, lactate content and ATP content were detected with spectrophotometry and bioluminescence method respectively. The conditions of cell apoptosis were measured with in situ- apoptosis assay kit. And cells transfected MCT1 antisense gene and A549 cells were inoculated in nude mouse subcutaneous to observe the growth of transplantation tumor. Results:Compared with A549 cells, the pHi of cells transfected MCT1 antisense gene was remarkably decreased (P<005), and lactate content was remarkably increased (P<0.001), ATP content was remarkably decreased (P<005). Apoptosis rate of cells transfected MCT1 antisense gene was remarkly higher than A549 cells(P<001). And the nude mouse transplantation tumors inoculated cells transfected MCT1 antisense gene were significantly lighter and smaller than that ones inoculated A549 cells(P<0.001). Conclusion: MCT1 gene plays an important role in proliferation and apoptosis of tumor cells.
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