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Inhibitory effect of intratumoral dendritic cells injection combined with radiotherapy on growth of renal carcinoma
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Abstract:
Objective: To study the effect of radiotherapy (RT) on dendritic cell (DC)-based immunotherapy of renal cancer in mice. Methods: Mice s.c. renal carcinoma models were established by transplanting Renca renal carcinoma cells and were divided into control group, single radiotherapy group, single DC injection group, and DC injection plus radiotherapy group. Mice in the last group received radiotherapy (7 Gy/time) on d 12-16 after inoculated with renal carcinoma cells (2.5×10 6); and on d 11, 15, 19 and 22, bone marrow-derived un-pulsed DCs (without tumor antigen,10 6 every time) were injected into the tumors of model mice. Mice were sacrificed on day 28 and the growth rate, weight and size of tumors were measured. IL-2, IFN-γ, IL-4 and IL-10 in the splenocytes were determined by ELISA assay.Results: Compared with single DCs and single RT group, DCs plus RT group had an obviously decreased growth rate and tumor size, but the splenocytes had an increased secretion of IL-2, IFN-γ and IL-4 compared with other groups (P<001). Conclusion: Intra-tumor DCs injection combined with radiotherapy can effectively inhibit the growth of renal carcinoma growth in BALB/c mice; its effect is better than any of the single strategy.
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