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Effect of basil polysaccharide on mRNA expression of tissue inhibitor of metalloproteinases in human breast cancer cell line cultured under hypoxia
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Abstract:
Objective： To investigate the effect of hypoxia on the mRNA expression of tissue inhibitor of metalloproteinases (TIMPs) in breast carcinoma cell line (MDA-231) and the effect of basil polysaccharide (BP) on the expression of TIMPs. Methods： MDA-231 cells were cultured under normoxia（21% O2, 5% CO2）, hypoxia（1% O2, 5% CO2 and 94% N2）or were treated with BP for 6 h separately. The expression of TIMP1, 2, 3 mRNA was detected by RT-PCR. Results： Expression of TIMP1, 2 mRNA, but not TIMP3 mRNA, was detected in MDA-231 cells. After cultured in hypoxia condition for 6 h, the expression of TIMP1 and TIMP2 mRNA increased significantly (P<0.05). The expression of TIMPs mRNA changed significantly in both hypoxia and normoxia group after BP treatment: the expression of TIMP1, 2 mRNA in normoxia group decreased significantly (P<0.05) and that in hypoxia increased significantly (P<0.05). Conclusion： BP has opposite influence on expression of TIMP1, 2 in human breast cancer cell line MDA-231 under hypoxia and normoxia conditions, indicating that in vitro study on anti-tumor drugs and bio-therapy should take the oxygen environment into consideration.
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