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Anti-prostate cancer activity of BH3-HIV-TAT
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Abstract:
Objective： To investigate the influence of BH3-HIV-TAT peptide on proliferation of prostate cancer cell line LNCap.Methods: LNCap cells were treated with BH3-HIV-TAT (25, 50, and 100 μmol/L) for 12 or 24 h. Then, laser scanning confocal fluorescence microscope was used to observe the cellular localization of BH3-HIV-TAT; Hoechst33258 staining was used to observe the morphological changes of LNCap cells during apoptosis; flow cytometry (FCM) was applied to study cell apoptosis and cell cycle of LNCap cells; and MTS/PMS assay was used to analyze whether the effect of BH3-HIV-TAT peptide was concentration dependent.Results: 〖WTBZ〗It was found that BH3-HIV-TAT peptide was mainly located in cell nucleus. The apoptosis of LNCap cells had 2 stages after BH3-HIV-TAT peptide treatment. Flow cytometry confirmed the peptide arrested cell cycle and induced apoptosis of LNCap cells. MTS/PMS assay showed BH3-HIV-TAT peptide had a concentration-dependent anti-LNCap cell effect. Conclusion: BH3-HIV-TAT peptide can effectively induce apoptosis of LNCap cells, influencing the proliferation of the cells, which provide an evidence for targeted therapy of cancer.
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