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Regulatory effect of interferon-γ on Fas/FasL system and apoptosis of leukemic cells
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Abstract:
Objective: To investigate the regulatory effect of interferon-γ on the expression of Fas/FasL and the apoptosis of leukemic cells. Methods: The expression of Fas and its function in human acute leukemic cell line HL-60 and in bone marrow mononuclear cells (BMMNC) of patients with acute myeloid leukemia (AML) were studied by immunohistochemical method, TUNEL method, and cell culture technique. The regulatory effect of interferon-γ on them was also investigated. Results: Compared with BMMNC of normal human, leukemic cells had an obviously decreased expression of Fas and induced apoptosis of Jurkat cells. It was found that interferon-γ up-regulated the expression of Fas in a time- and dose-dependent manner（P<0.01）. Meanwhile, interferon-γ inhibited Jurkat cells apoptosis induced by leukemic cells, increased the Fas-mediated apoptosis of leukemic cells, and increased the sensitivity of leukemic cells to chemotherapy agents.Conclusion: Interferon-γ can prevent the immune escape of leukemic cells via regulating the Fas/FasL system and increase the sensitivity of leukemic cells to the chemotherapy drugs targeting Fas/FasL system.
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