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Preparation of functional antibody for inhibition of adhesion between esophageal carcinoma and pulmonary vascular endothelia
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Abstract:
Objective: To prepare monoclonal antibody for inhibition of adhesion between esophageal carcinoma and pulmonary vascular endothelia, so as to pave a way for the targeted treatment of esophageal carcinoma metastasis.Methods: Tumor cells were separated from esophageal carcinoma tissues and were used to immunize Balb/c mouse. The spleen cells of the immunized mouse were fused with SP2/0 cells. The anti-adhesion antibodies were then screened and identified by ELISA, immunohistochemistry, immunofluorescence, tumor-endothelial cell adhesion assay, and Western blotting. Results: Totally 1 134 clones of monoclonal antibodies were obtained after fusion. Among the 486 clones which could interact with the membrane of the esophageal carcinoma, 294 clones had no or weak reactions with normal esophageal epithelia. The 15 selected clones significantly suppressed the adherence between esophageal cancer cells and lung endothelia. Western blotting displayed that 2 of the clones reacted with the antigens having a molecular weight of 30 000 and 60 000, respectively.Conclusion: We have successfully obtained several clones of functional monoclonal antibodies which can suppress the adherence of esophageal carcinoma cells with lung micro-vascular endothelia, and 2 of the clones show promise in anti metastasis therapy of esophageal carcinoma.
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