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Inhibition of allogeneic T-cell proliferation by indoleamine 2,3-dioxygenase-modified dendritic cells
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Abstract:
Objective: To study the inhibitory effect of indoleamine 2,3-dioxygenase(IDC)-modified dendritic cells on proliferation responses of allogeneic T-cell in transplantation of the hematopoietic stem cells in vitro. Methods： BALB/c mice bone marrow-derived dendritic cells were transfected with recombinant adenovirus harboring AdIDO gene. IDO mRNA was detected by RT-PCR in DCs transfected with AdmIDO and the changes of DCs phenotype were analyzed by FACS. Naive T lymphocytes from the spleen of C57BL/6 mice were co-cultured with BALB/c mouse-derived IDO-DC and Mixed lymphocyte reaction were performed to evaluate the alloantigen-specific hyporesponsivness and T cell apoptosis.
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