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Interleukin-27 inhibits tumor angiogenesis through upregulating expression of MIG and IP10
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Abstract:
Objective: To study the inhibitory effect of IL27 against human tumor angiogenesis and the related mechanisms. Methods: Human esophageal carcinoma cells (Eca109/IL27) stably transfected with IL27 gene were injected into nude mice to establish tumorbearing mouse model. The survival time and tumor growth were observed. IFNγ level secreted by splenocytes was measured by ELISA. Expression of VEGF and CD34 was detected by immunohistochemistry method and MVD was calculated according to CD34 level. RTPCR was used to detect the expression of IP10 and MIG mRNA in the tumor tissues. Results: The survival time of mice injected with Eca109/IL27 cells was significantly longer than those of mice injected with wide type Eca109 or Eca109/LXSN (blank vector) cells (P<0.05). Expression of VEGF and CD34 in Eca109/IL27inoculated mice was lower than those in Eca109 or Eca109/LXSN groups. Production of IFNγ by splenocytes in mice injected with Eca109/IL27 cells was higher than those with Eca109 and Eca109/LXSN (P<0.05). MIG and IP10 mRNA expression was also higher than those in Eca109 or Eca109/LXSN injected mice (P<005). Conclusion: IL27 can inhibit tumor angiogenesis in nude mice through upregulating the expression of MIG and IP10, and thus exerts antitumor effect
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Supported by the Key Science and Technology Research Project of Chinese Technology Administration（No. 2006BAI02A07）
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