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Basic Research
of GM-CSF membranemodified melanoma cell B16.F10 vaccine against implanted tumors in mice
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Abstract:
Objective:To study the inhibitory effect of GMCSF（granulocytemacrophage colony stimulating factor) membraneanchored B16.F10 (murine melanoma cell line) vaccine against implanted tumors in mice. Methods: GMCSF membraneanchored B16.F10 vaccine was prepared by fusing biotinavidinGMCSF together. BALB/c mice were randomly divided into 5 groups: GMCSF membraneanchored B16.F10, B16.F10 mixed with GMCSF, B16.F10 cells, GMCSF and 0.9% sodium chloride. At day 1 and 7, all BALB/c mice were immunized; at day 14, all the mice received subcutaneous injection of 0.2 ml wildtype B16.F10 cells (1×106 cells). Tumorfree rate and survival time of different groups were measured. INFγ levels in splenocytes were detected by ELISA at day 24 in different groups.Results: Sixty days and 90 d after subcutaneous injection with B16.F10 cells, tumorfree rate and survival rate of mice in GMCSF membraneanchored B16.F10 group, B16.F10 mixed with GMCSF group, B16.F10 cell group were 0% and 0%, 100% and 100%; 50% and 40%, 70% and 40%; 20% and 20%, and 80% and 20%, respectively. The tumorfree rate and survival rate of mice in GMCSF group and 0.9% sodium chloride group were both 100% and 0%, respectively. INFγ levels in splenocytes in GMCSF membraneanchored B16.F10 group were significantly higher than those of other groups（P<0.01）. Conclusion: GMCSF membraneanchored B16.F10 vaccine can induce specific antitumor immunity in mice and can prevent later B16.F10 tumor cells challenge.
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Project Supported:
Surpported by the Natural Science Key Program of Guangdong Province(No.06104396)
Clc Number:
