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Expression features of P2X family receptors in acute T lymphoblastic leukemia mouse model
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Abstract:
Abstract Objective:To investigate the expression features of P2X family receptors during the development and progression of murine acute T lymphoblastic leukemia. Methods: A Notch1 over-expressing murine T cell induced-acute lymphoblastic leukemia model was prepared. CD45.2+GFP+ leukemia cells were sorted by flow cytometry. The expressions of P2X family receptors were determined by real-time PCR. P2X7 receptor-mediated intracellular Ca 2+ change was measured by fluorescent spectrophotometer. Results: Mouse bone marrow cells (BMNCs) of both control and leukemia mice expressed all P2X receptors except for P2X5. P2X7 receptor expression increased gradually during the induction of T lymphoblastic leukemia; P2X1 and P2X3 receptors decreased; and P2X2, P2X4 and P2X6 receptors had no detectable changes. Similar expression patterns were observed in sorted CD45.2+GFP+ leukemia cells. P2X7 receptor-mediated calcium response was detected in BMNCs from both leukemia and control mice; and the response could be blocked by its specific antagonist KN62. However, the calcium response showed different patterns: it sustained an increase in leukemia group, whereas gradually decreased after reaching peak in the control group. Conclusion: P2X1, P2X3 and P2X7 receptor expressions are related to the development and progression of murine acute T lymphoblastic leukemia, suggesting that intercellular communications mediated by these receptors may play important roles in leukemia.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30570770), and the Natural Science Foundation of Tianjin (No. 06YFJMJC15700)
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