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Oridonin induces apoptosis of human multiple myeloma ARH-77 cells and its possible mechanism
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Abstract:
Abstract Objective:To investigate the apoptosis induction effect of oridonin (Ori) on human multiple myeloma cell line ARH-77 and the possible mechanism. Methods: ARH-77 cells were treated with different concentrations of oridonin (2.5, 5 and 10 μmol/L), and then the proliferation of ARH-77 cells was detected by MTT assay. The morphological change of apoptotic ARH-77 cells was observed under phase contrast microscope and Hoechst 33258 staining. The apoptosis and the change of mitochondrial membrane potential (Δψm) of ARH-77 cells were examined by flow cytometry. The caspase 8 activity was measured by caspase-8 apoptosis kit. Results: Ori significantly inhibited the growth of ARH-77 cells in a time- and dose-dependent manner. After treatment with 10 μmol/L Ori for 24 h, ARH-77 cells became shrunk, with cytoplasm vacuoles and apoptotic bodies. Flow cytometry results revealed that the apoptotic rates of ARH-77 cells after Ori treatments (2.5, 5 and 10 μmol/L) were significantly higher than that of control group, with apoptotic rates being (15.07±0.78)%, (21.00±1.49)%, (27.45±2.47)% vs (5.27±1.46)% (P<0.01). Green fluorescence percentages of Δψm of ARH-77 cells were significantly increased in Ori-treated groups (2.5, 5 and 10 μmol/L) compared with that in control group, with percentages being (26.80±0.75)%, (36.81±2.27)%, (49.48±1.10)% vs (16.96±0.50)% (P<0.01). Meanwhile, caspase 8 activity of ARH-77 cells was significantly up-regulated by Ori treatment (P< 0.01). Conclusion: Oridonin can markedly induce apoptosis of human multiple myeloma ARH-77 cells, which might be related to extrinsic and intrinsic pathways.
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Project Supported:
Project supported by the Natural Science Foundation of Guangdong Province (No.7001196)
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