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Trichostatin A up-regulates expression of Coxsachievirus and adenovirus receptor in human esophageal cancer EC1 cells through inhibiting MAPK/ERK pathway
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Abstract:
Abstract Objective:To observe the effect of trichostatin A (TSA) on Coxsachievirus and adenovirus receptor (CAR) expression in membrane of human esophageal cancer EC1 cells, and to discuss the role of MAPK/ERK signal pathway in the up-regulation of CAR expression triggered by TSA. Methods: EC1 cells were treated with TSA (0.3, 0.5, 1.0 μmol/L), and CAR expressions on EC1 cells were examined by immunofluorescence staining, RT-PCR and Western blotting analysis. EC1 cells were also treated with 1.0 μmol/L TSA for 0, 1, 6, 12, 24, and 48 h, and then the CAR expression and phosphorylation of ERK were detected by Western blotting analysis. The correlation between ERK phosphorylation level and the CAR expression was analyzed. Results: CAR protein and mRNA expressions in EC1 cells were significantly increased after treatment with 0.3, 0.5, and 1.0 μmol/L TSA (P<0.05), and the increase was in a dose-dependent manner. EC1 cells treated with 1.0 μmol/L TSA for different time periods also showed significantly increased CAR expression (P<0.05), while p-ERK expression levels in EC1 cells were remarkably decreased. The expression of p-ERK in EC1 cells treated with TSA was negatively correlated with that of CAR (r=-0.886, P<0.01). Conclusion: TSA can increase the expression of CAR in human EC1 cells, and the possible mechanisms may be related to the inhibition of ERK/MAPK pathway in EC1 cells.
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Project Supported:
Project supported by the Key Foundation of Science and Technology Innovation Program of Education Ministry of China (No. 207150)
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