Mobile website
Basic Research
KiSS-1 inhibits invasion and migration of osteosarcoma MG63 cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Abstract Objective:To investigate the effects of tumor metastasis-suppressor gene KiSS-1 on the invasion and migration abilities of osteosarcoma MG63 cells. Methods: KiSS-1 expression plasmid pSNAV2.0-KiSS-1 was constructed and transfected into MG63 cells. MG63 cells stably transfected with pSNAV2.0-KiSS-1 (named MG63-KiSS-1) were selected by G418. KiSS-1 mRNA and protein expression in MG63-KiSS-1 cells was examined by real-time PCR and Western blotting analysis, respectively. The invasion ability of MG63 cells was detected by transwell assay. The effects of KiSS-1 on the invasion and migration abilities of MG63 cells were measured by millicell assay and cell scratch healing assay. Results: MG63 cells stably transfected with pSNAV2.0-KiSS-1 were successfully established. MG63-KiSS-1 cells highly expressed KiSS-1 protein. The invasion ability of MG63 cells was significantly decreased after pSNAV2.0-KiSS-1 transfection (P<0.05). The migration ability of MG63 cells was also significantly inhibited after pSNAV2.0-KiSS-1 transfection as examined by millicell assay and cell scratch healing assay (P<0.05). Conclusion: KiSS-1 gene can significantly inhibit the invasion and migration abilities of osteosarcoma MG63 cells, which may play a key role in metastasis of osteosarcoma.
Keywords:
Project Supported:
Project supported by the Joint Scientific Research Foundation of Ministry of Health and the Health and Education Department of Fujian Province (No.WKJ2008-2-042)
Clc Number:
