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Foxp3 expression in neuroblastoma cell line SK-N-SH and its sensitivity to chemotherapy
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Abstract:
Abstract Objective: To investigate Foxp3 expression in neuroblastoma cell line SK-N-SH and its chemosensitivity to cyclophosvnamide (CTX) and pirarubicin (THP). Methods: SK-N-SH cells were cultured in vitro, and Foxp3 expression in SK-N-SH cells was examined by flow cytometry (FCM). The sensitive dosages of CTX and THP on SK-N-SH cells were determined by MTT assay. The effects of CTX or THP on Foxp3 expressions in SK-N-SH cells were examined by FCM and real-time PCR. Results: FCM results showed that SH-N-SK cells expressed high level of Foxp3. The sensitive dosage of CTX on SK-N-SH cells was 6 mmol/L, and that of THP was 80 ng/ml. CTX (6 mmol/L), THP (80 ng/ml) alone or in combination could not inhibit the expression of Foxp3 in SK-N-SH cells (P>0.05). Real-time PCR data also showed that CTX, THP alone or in combination could not down-regulate the expression of Foxp3 in SK-N-SH cells at mRNA level (P>0.05). Conclusion: Neuroblastoma SK-N-SH cells can express high level of Foxp3, but Foxp3 shows no chemosensitivity to CTX and THP.
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Project Supported:
Project supported by the National High Technology Research and Development Program of China (863 Program) (No. 2007AA021004)
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