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Analysis of micronucleus in peripheral blood lymphocytes of leukemia patients
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Abstract:
Objective:To study the genetic damage of preliminarily diagnosed leukemia patients by micronucleus analysis technology. Methods: Peripheral blood of 54 preliminarily diagnosed leukemia patients (11 CML patients, 7 AML-M1 patients, 6 AML-M2 patients, 4 AML-M3 patients, 2 AML-M4 patients, 4 AML-M5 patients, 2 AML-M6 patients, and 18 ALL patients) and 30 healthy volunteers were collected and examined by cell cycle block test. The genetic damages of patients were assessed by micronucleus rate (MNR), micronucleus cell rate (MCR), nuclear bud (Bud) frequency, nucleoplasmic bridge (NPB) frequency, nuclear division index (NDI), and apoptotic cell (AC) rate combined with chromosome metaphase, gene fusion and gene rearrangement analysis. Results: The chromosome damages in 54 leukemia patients were significantly different from those in the 30 healthy volunteers, with MNR being (17.368±1.305)‰ vs (7368±0.844)‰, MCR being (15.418±1.212)‰ vs (5.887±1.101)‰, Bud frequency being (8.142±0132)% vs (0.404±0.404)%, NPB frequency being (5.724±0.874)% vs (0.034±0.034)%, NDI being (1.722±0.062)% vs (2.282±0.324)%, AC rate being (2.167±0.333)% vs (0.167±0.667)%, abnormal chromosome detection rate being 24.00%, gene fusion or gene rearrangement positive rate being 18.00% (P<0.05 or P<001). Conclusion: Leukemia patients show different degrees of inheritance chromosome damages in the initial stage, indicating that the inheritance damage caused by chromosomal instability is related to leukemia pathogenesis.
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Project Supported:
Project supported by the Technology Research and Development Special Foundation of Gansu Province (No.CYA013)
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