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Detection of CD45low CD34+ KDR+ endothelial progenitor cells in laryngeal squamous carcinoma patients and their clinical significance
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Abstract:
Objective: To study the ratio of circulating CD45low CD34+ KDR+ endothelial progenitor cells (EPCs) in peripheral blood of laryngeal squamous carcinoma patients, and to investigate its relationship with the development and progress of laryngeal squamous carcinoma. Methods: Blood samples from 20 laryngeal squamous carcinoma patients, who were diagnosed from Feb. 2010 to Feb. 2011, and 10 healthy volunteers were included in the present study. The ratio of CD45low CD34+ KDR+ EPCs in peripheral CD34+ haemopoietic stem cells or peripheral lymphocytes was detected by flow cytometry, the expressions of KDR and CD133 mRNA in PBMC were examined by real-time RT-PCR, and the levels of VEGF and NO in plasma were assayed by ELISA and Griess assay, respectively. Results: The ratios of CD45low CD34+ KDR+EPCs in both peripheral CD34+ haemopoietic stem cells and peripheral lymphocytes of laryngeal squamous carcinoma patients in stage Ⅱ-stage Ⅳ were significantly higher than those in healthy volunteers (\[55.4±11.4\]% vs \[21.0±5.8\]%，P<0.05; \[1.27±0.25\] vs \[0.25±0.09\], P<0.05). The expressions of KDR and CD133 mRNA and the level of plasma VEGF in laryngeal squamous carcinoma patients of stage Ⅱ-stage Ⅳ were significantly higher than those in healthy volunteers (P<0.05), but there was no significant difference in NO level. The ratio of CD45low CD34+ KDR+EPCs, the expression of KDR mRNA and CD133 mRNA, and the level of plasma VEGF in laryngeal squamous carcinoma patients of stageⅠwere similar to those in healthy volunteers. Conclusion: The ratio of circulating CD45low CD34+ KDR+ EPCs in peripheral blood and serum VEGF in advanced laryngeal squamous carcinoma patients are increased, which may be related to the development of tumors.
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Project supported by the Science Foundation for Young Scholars from Health Bureau of Shanghai (No. 2007Y43)
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