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Inhibitory effect of Brucea javanica oil emulsion on proliferation of NCIH460 cells and underlying mechanism
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Abstract:
Objective: To investigate the inhibitory effect of Brucea javanica oil emulsion on the growth of human largecell lung cancer NCIH460 cells in vitro and its mechanisms. Methods: NCIH460 cells were treated with different concentration of Brucea javanica oil emulsion (2.5, 5.0, 10, 20, 40 and 80 μg/ml) for 24, 48, and 72 h. The inhibitory effects of Brucea javanica oil emulsion on proliferation of NCIH460 cells was examined by MTT; morphological changes was observed by optical microscope after Giemsa staining; apoptotic rate of NCIH460 cells was examined by flow cytometry; and caspase3 activity in NCIH460 cells was detected with caspase3 activity kit. Results: Brucea javanica oil emulsion significantly inhibited the proliferation of NCIH460 in a dose and timedependent manner, and the inhibiting rate reached (91.07±1.60)% after 80 μg/ml Brucea javanica oil emulsion treatment for 72 h. Typical apoptotic morphological changes also appeared after Brucea javanica oil emulsion treatment. Flow cytometry results demonstrated that the apoptotic rates of NCIH460 cells were (45.23±4.30)%, (54.14±3.09)% and (61.57±7.28)% after 10, 20 and 40 μg/ml Brucea javanica oil emulsion treatment for 48 h, respectively (P＜0.01). In NCIH460 cells, the Brucea javanica oil emulsion increased the activity of caspase3 in a dosedependent manner. after 40 μg/ml Brucea javanica oil emulsion treatment, caspase 3 activity reached 1.07±0.07 μmol/μg, which was 4.97 fold higher than control group (P＜0.01). Conclusion:Brucea javanica oil emulsion can induce apoptosis and inhibit the proliferation of NCIH460 cells, possibly through increasing caspase3 activity.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30670916), and the Science and Technology Program of Science and Technology Bureau of Shenyang (No. 1063315100)
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