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Clinical Significance of circulating endothelial progenitor cells counting in hepatocellular carcinoma
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Abstract:
Objective:To investigate the clinical significance of circulating endothelial progenitor cells (EPCs) counting in diagnosis and prognosis of hepatocellular carcinoma (HCC). Methods: Tirtynine patients with HCC and 20 healthy individuals were recruited from Shanghai First People’s Hospital Baoshan Branch (Jan. 2008 to Dec. 2010) in this study. Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation, then stained with antibodies against CD34, CD133, VEGFR2 which were conjugated to FITC, PE and APC respectively. Circulating EPCs were characterized as CD34+CD133+VEGFR2+ cells and its proportion in peripheral blood mononuclear cells was detected by flow cytometry. Relationship between the proportion of CD34+CD133+VEGFR2+ EPC and clinical stage, etc. of HCC was analyzed. Results:The level of circulating CD34+CD133+VEGFR2+ EPCs in HCC patients was significantly higher than that in healthy control(0.138±0.05)%,vs (0.027±0.013)% (P<0.05). Furthermore, the level of circulating EPCs in HCC patients was positively correlated to clinical stages. Conclusion: Circulating CD34+CD133+VEGFR2+ EPCs proportion is upregulated in HCC patients, and related to clinical stage of HCC, which may be significant in diagnosis and prognosis of HCC.
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Project Supported:
Project supported by the Science Foundation for Young Scholars of Shanghai Health Bureau (No. 2007Y43)
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