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Archive > Volume 20 Issue 2 > 2013,20(2):138-144. DOI:10.3872/j.issn.1007-385X.2013.02.002 Prev Next
Prompt Report
Preparation of silica-coated gold nanocluster and its application in dual mode imaging targeting gastric cancer cells and cancer xenograft
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Abstract:
Objective:To investigate the feasibility of targeted fluorescent imaging to MGC-803 gastric cancer cells and the potential of CT imaging of gastric cancer using nanocomposite probes of silica-coated gold nanocluster (AuNC@SiO2). Methods: The AuNC was coated by multilayer silica shells to obtain AuNC@SiO2. AuNC@SiO2 was conjugated with cancer cell targeted folic acid (FA), and AuNC@SiO2-FA was then used to investigate the feasibility of targeted fluorescent imaging to gastric cancer cells. MG-803 cells were injected subcutaneously into BABL/c nude mice to establish a cancer xenograft model. CT imaging was performed to collect CT signals before and after subcutaneously injection of AuNC@SiO2, thus exploring the feasibility of AuNC@SiO2 nanoparticles in CT imaging of tumor mouse models. Results: AuNC@SiO2 was successfully constructed with a uniform shape, good dispersbility and fluorescence optical properties. AuNC@SiO2 and AuNC@SiO2-FA showed a low cytotoxicity on gastric cancer MGC-803 cells and normal gastric mucosal GES-1 cells at a concentration of 500 mg/ml. The AuNC@SiO2-FA showed an effectively targeted ability to gastric cancer MGC-803 cells rather than normal GES-1 cells, thus presenting a clearly fluorescence imaging. CT signals were significantly enhanced in tumors after AuNC@SiO2 injection, with HU value increasing from 129.16 before injection to 383.32. Conclusion: AuNC@SiO2 can be used for fluorescence imaging and CT dual mode imaging. Moreover, AuNC@SiO2 conjugated with FA can realize gastric cancer cells targeted fluorescence imaging.
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Project Supported:
Project supported by the National Key Basic Research Development Program (973 Program) of China (No.2010CB933901)，and the National Science Foundation for Distinguished Young Scholars of China (No.881225010)
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