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Archive > Volume 20 Issue 2 > 2013,20(2):145-152. DOI:10.3872/j.issn.1007-385X.2013.02.003 Prev Next
Prompt Report
Targeted migration and killing effect of mesenchymal stem cells infected with Lenti-TK on CD133+ cancer stem cells in nasopharyngeal carcinoma
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Abstract:
Objective: To investigate the targeted migration and killing effect of mesenchymal stem cells (MSCs) infected with Lenti-TK (thymidine kinase) vector on CD133+ cancer stem cells in nasopharyngeal carcinoma. Methods: The recombinant lentiviral expression vector containing TK gene (Lenti-TK) was constructed and transduced into MSC (Lenti-TK-MSC). Fusion tag-TK (HA-TK) expression was verified by RT-PCR and Western blotting. CD133+ cells were sorted from nasopharyngeal carcinoma 5-8F cells by immunomagnetic beads. The chemotactic ability of Lenti-TK-MSC to CD133+5-8F cells was analyzed by Transwell assay. The CD133+ 5-8F cells were co-cultured with Lenti-TK-MSC and GCV to detect its killing effect on cells by CCK-8 Kit. Results: The recombinant lentivirus vector Lenti-TK was successfully constructed with titer being 1×108 UT/ml. The transduction efficiency of Lenti-TK to MSC was (95.1±0.1)%, 72 h after transduction at an MOI of 50. The migration number of Lenti-TK-MSC to CD133+5-8F cells was more than that to CD133-5-8F cells and 5-8F cells (\[83.0±8.7\] vs \[29.6±5.3\] vs \[38.3±5.2\]；P=0.000). The Lenti-TK-MSC/GCV treatment significantly inhibited the growth of the CD133+ 5-8F cells compared with the GCV group and the Lenti-TK-MSC/GCV condition medium group (the culture supernatant of Lenti-TK-MSC treated with 1 mg/L GCV for 48 h) (\[37.2±2.3\]% vs \[98.5±3.1\]% vs \[83.8±3.4\]%, P=0.000), with the cell survival being (68.2±2.3)% when the proportion of Lenti-TK-MSC was 20%, which showed a significant bystander killing effect. Conclusion: Lenti-TK infected MSC exert targeted migration and killing effects on CD133+ 5-8F cells from nasopharyngeal carcinoma.
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Project Supported:
Project supported by the Science and Technology Plan in Guangdong Province of China（No.2010B031200009)
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