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Archive > Volume 20 Issue 2 > 2013,20(2):177-181. DOI:10.3872/j.issn.1007-385X.2013.02.008 Prev Next
Basic Research
Silencing Med19 expression on proliferation and apoptosis of colon cancer Caco-2 cells
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Abstract:
Objective: To explore the effect of the down-regulation of Med19 expression by RNAi on the proliferation and apoptosis of colon cancer Caco-2 cells. Methods: The pSilencer-Med19-siRNA interference plasmid targeting Med19 was constructed, which was transfected into Caco-2 cells. The level of Med19 mRNA in transfected Caco-2 cells was detected by RT-PCR and the level of Med19 protein was determined by Western blotting. Flow cytometry and MTT were performed to detect the proliferation and apoptosis of Caco-2 cells after pSilencer-Med19-siRNA transfection. Results: The results of RT-PCR and Western blotting showed that the mRNA and protein levels of Med19 declined markedly in Caco-2 cells that were transfected by pSilencer-Med19-siRNA vector (P<0.01). Compared with the control pSilencer group, flow cytometry and MTT assay demonstrated that pSilencer-Med19-siRNA significantly suppressed proliferation （7 d: \[086±0.09\]% vs \[1.38±1.10\]%, P<0.01) and induced the apoptosis of Caco-2 cells (\[22.72±2.85\]% vs \[723±1.29\]%, P<0.01) in the pSilencer-Med19-siRNA group. Conclusion: Silence Med19 expression by interference RNA can inhibit the proliferation and induce apoptosis of colon cancer Caco-2 cells, so Med19 may act as a potential therapeutic target in colon cancer.
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