Mobile website
Archive > Volume 20 Issue 2 > 2013,20(2):197-200. DOI:10.3872/j.issn.1007-385X.2013.02.012 Prev Next
Basic Research
IL-12 enhances the cytotoxicity of cytokine-induced killer cells against esophageal carcinoma EC9706 cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To explore the effects of IL-12 on the phenotypes of cytokine-induced killer (CIK) cells and the cytolytic activity of CIK cells against human esophageal carcinoma EC9706 cells in vitro. Methods: Peripheral blood mononuclear cells were isolated from healthy donors. Two groups were designed: a control group (cells were cultured in the presence of IFN-γ, IL-2 and anti-CD3 antibody) and IL-12 group (cells were cultured in the presence of IFN-γ, anti-CD3 antibody , IL-2 and IL-12 ). After 14-day culture, the phenotypes of CIK cells in the control and IL-12 groups were analyzed by flow cytometry. The cytotoxic activity of CIK cells on EC9706 cells was measured by LDH releasing assay at effect-to-target (E ∶T) cell ratios of 20 ∶1, 30 ∶1. The anti-NKG2D monoclonal antibody was added to CIK cells to detect its effect on the cytotoxic activity of CIK cells at E ∶T ratio of 30 ∶1. Results: In comparison to the control group, the proportion of CD3+CD56+cells, NKG2D expressions on CD3+ and CD3+CD56+cells, and perforin expression in CD3+ cells were higher in the IL-12 group (\[28.23±1.71\]% vs \[16.34±0.59\]%; \[77.45±2.15\]% vs \[66.87±0.73\]%,\[92.94±0.77\]% vs \[82.18±0.66\]%; \[51.78±0.63\]% vs \[43.54±095\]%; all P<0.05). Whereas, no significant change was observed in the granzyme B expression in the CD3+ cells \[(26.90±0.67）% vs (26.76±033）%，P>0.05\]. The cytolytic activity of CIK cells against EC9706 cells was increased significantly in the IL-12 group (E ∶T ratio of 20 ∶1, \[43.92± 1.67\]% vs \[35.34±1.22\]% ; E ∶T ratio of 30 ∶1, \[55.95±0.88\]% vs \[43.91±1.10\]%, all P<0.05). The cytotoxicity of CIK cells in the IL-12 and the control groups were significantly inhibited by anti-NKG2D monoclonal antibody (\[19.72±0.56\]% vs \[55.95±0.88\]%, \[19.83±1.20\]% vs \[43.91±1.10\]%, all P<0.05). Conclusion: IL-12 up-regulates the NKG2D and perforin expressions on CIK cells, enhancing their cytotoxicity against EC9706 cells.
Keywords:
Project Supported:
Project supported by the Science and Technology Program of Henan Province (No. 201143)
Clc Number:
