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Archive > Volume 20 Issue 2 > 2013,20(2):212-216. DOI:10.3872/j.issn.1007-385X.2013.02.015 Prev Next
Basic Research
Comparison of antitumor effect of Newcastle disease virus HBNU/LSRC/F3 strain to ECA109 cells with Mukeswar and LaSota strains
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Abstract:
Objective:To explore the impact of Newcastle disease virus (lentogenic HBNU/LSRC/F3) strain on apoptosis of esophageal cancer ECA109 cells, and to compare with the other two strains (lentogenic LaSota strain and mesogenic Mukteswar strain) of their anti-tumor effects. Methods: ECA109 cells were cultured in vitro and infected by three NDV strains respectively. The proliferation inhibiting effects of NDVs on ECA109 cells were detected by CCK-8. The apoptosis of ECA109 cells after NDV infection was detected by laser confocal microscopy. Flow cytometry and agarose gel electrophoresis were used to detect the early and late apoptosis of ECA109 cells, respectively. Results: HBNU/LSRC/F3 NDV strain suppressed the proliferation of ECA109 cells, which was stronger than LaSota strain and weaker than Mukteswar strain (P<0.05). The proliferation inhibition rates of HBNU/LSRC/F3 strain, Mukteswar strain and LaSota strains on ECA109 cells were (39.87±1.99)%, (31.43±1.57)% and (19.89±0.99)%, respectively, infected by 5×10-4 Hu/ml NDV. Flow cytometry showed that the early apoptosis rate of ECA109 cells infected by HBNU/LSRC/F3 strain was (21.32±0.44)%, while Mukteswar strain was (22.27±0.23)% and LaSota strain was (14.32±0.61)%. Laser scanning confocal microscopy and DNA gel electrophoresis showed that Mukteswar strain primarily induced the late apoptosis. Whereas, HBNU/LSRC/F3 strain primarily induced the early apoptosis, which was more obvious than LaSota strain. Conclusion: Lentogenic HBNU/LSRC/F3 strain can effectively inhibit ECA109 cell proliferation and induce early apoptosis of ECA109 cells, which was lower than Mukteswar strain and significantly higher than LaSota strain. Therefore, HBNU/LSRC/F3 strain may have a high clinical value for anti-tumor therapy.
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Project Supported:
Project supported by the Natural Science Foundation of Hebei Province（No. C2010001450）
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