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Archive > Volume 20 Issue 2 > 2013,20(2):230-236. DOI:10.3872/j.issn.1007-385X.2013.02.018 Prev Next
Clinical Research
CD90+ cancer stem cells may influence metastasis and prognosis of gastric cancer
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Abstract:
Objective:To isolate and identify the cancer stem cells from gastric cancer cell line SNU-5, and investigate the influence of CD90+ cancer stem cells on the metastasis and prognosis of gastric cancer. Methods: Non-adherent culture and PKH26 staining was used to determine whether there were cancer stem cells in human gastric cancer cell line SNU-5; Flow cytometry analysis was performed for identification of positive cells with stem cell markers within parent SNU-5 cells and sphere cells; CD90+ SNU-5 cells were sorted to identify its biological characteristics in vivo and tumorigenicity in vitro. Ninety-five cases with gastric cancer selected from cancer Hospital, immunohistochemistry was applied to detect CD90 expression in human gastric cancer. Results: The single PKH26 positive cell was observed in spheroids after SNU-5 cells were cultured for 11 days and CD90 could be co-stained with PKH26 in the spheroid cells, a dye indicating the characterization of stem cells. Serum sphere culture can enrich CD90+ SNU-5 cells by 6.1 times. The sorted CD90+ SNU-5 cells presented higher self-renewal ability (sphere formation rate: \[7.7±1.1\]% vs \[1.3±0.4\]%, \[18±03\]%，P<0.01), higher invasion (the number of invasive cells \[283.3±30.2\] vs \[48.0±7.5\], \[156.7±7.2\], P<0.01)than CD90- SUN-5 cells and parent SNU-5 cells. Only 2×102 CD90+ SNU-5 cells can form tumors at 6 weeks (4/6) in severe combined immune deficency (SCID) mice, whereas at least 2×104 CD90- SNU-5 cells were needed to form tumors at 10 weeks (1/6). Immunohistochemical results showed that CD90 expression was significantly associated with distant metastasis of gastric cancer (P<0.01). The survival rate of CD90 positive gastric cancer patients is obviously lower than that of CD90 negative patients (P<0.01). Conclusion: CD90+ cancer stem cells in huamn gastric cancer cell line SNU-5 show increased self-renewal and invasion abilitities, which are related to metastasis and prognosis of gastric cancer.
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Project supported by the Major National Science and Technology Project of China (No. 2011ZX09102-010-02)
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