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Archive > Volume 20 Issue 6 > 2013,20(6):661-666. DOI:10.3872/j.issn.1007-385X.2013.06.005 Prev Next
Basic Research
BCSC-1 overexpression inhibited the proliferation, invasion, adhesion and migration of hepatocellular carcinoma Bel-7402 cells
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Abstract:
Objective:To study the effects of overexpression of BCSC-1 gene on the proliferation, invasion, adherence and migration abilities of hepatoma carcinoma Bel-7402 cells, and to investigate the possible mechanism. Methods: Bel-7402 cells were transfected by pcDNA3.1/v5-HisB-BCSC-1 and pcDNA3.1/v5-HisB respectively as BCSC-1 group and empty vector group, and the wild-type Bel-7402 cells as a wild group. Proliferation, invasion, adherence and migration abilities of the Bel-7402 cells were detected by MTT assay, Transwell assay, adhesion experiment in vitro and scratch test, respectively. Real-time PCR was performed to detect the expression of BCSC-1 and osteopontin (OPN), ICAM-1, PTTG mRNA, which were correlated with the cell growth and adhesion in Bel-7402 cells. Results: After transfected by pcDNA3.1/v5-HisB-BCSC-1, the expression of BCSC-1 mRNA in Bel-7402 cells was significantly raised compared with that of empty vector group and the wild-type group (\[10.58±0.56\] vs \[1.10±0.22\], \[1.00±0.01\]; all P＜0.01). The Bel-7402 cell line stably overexpressing BCSC-1 was successfully established. Compared with the empty vector group and wild group, the growth rate of Bel-7402 cells in the BCSC-1 group was obviously slower (72 h: \[0.29±0.003\] vs \[0.34±0.014\], \[0.35±0.013\]; all P<0.05), the invasion rate (\[76.20±1.85\]% vs \[93.42±3.24\]%, \[10000±105\]%; all P<0.01) and the adhesion rate (\[58.57±0.84\]% vs \[97.14±0.84\]%, \[100.00±130\]%; all P<0.01) were obviously decreased, the migratory distance was significantly reduced (\[116.60±10.58\] vs \[231.33±1026\], \[237.96±11.58\] μm; all P<0.01\]. The expression of OPN mRNA in the Bel-7402 cells which the BCSC-1 was up-regulated was significantly decreased (\[0.12±0.06\] vs \[0.95±0.14\], \[1.00±0.08\]; all P<001). No significant changes were observed in the expression of ICAM-1 and PTTG mRNA. Conclusion: Overexpression of BCSC-1 can inhibit the proliferation, invasion, adherence and migration abilities of Bel-7402 cells, which may be related to the decreased expression of OPN.
Keywords:
Project Supported:
Project supported by the Natural Science Foundation of Shandong Province （No.2009CM019）, and the Students Research Foundation of Weifang Medical College（No.KX2012009）
Clc Number:
