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Archive > Volume 20 Issue 6 > 2013,20(6):673-678. DOI:10.3872/j.issn.1007-385X.2013.06.007 Prev Next
Basic Research
Effects of down-regulation of lentivirus-mediated GnTⅣa on the proliferation and invasion of mouse hepatoma Hca-F cells
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Abstract:
Objective:To investigate the effect of down-regulation of lentivirus-mediated N-acetylglucosaminyltransferase Ⅳa (GnTⅣa) on the proliferation and invasion of mouse hepatoma Hca-F cells in vitro. Methods: Expression plasmid pll3.7/Mgat4a-shRNA with interference shRNA sequences gene target Mgat4agene, were encoding GnTⅣa was constructed. The RNAi lentiviral particles were packaged to infect Hca-F cells. The effect of Mgat4a-shRNA infection on the Mgat4a mRNA and GnTⅣa expression of Hca-F cells was detected by RT-PCR and flowcytometry, respectively. The effect of recombinant lentiviral infection on the proliferation and invasion capability of Hca-F cells was determined by CCK-8 and transwell assay in vitro, respectively. Results: The mouse hepotoma Hca-F cells could be infected by the lentivirus with a high efficiency, and the infection efficiency of Mgat4a-shRNA group reached 72%. Compared with the Hca-F cells and Mock-shRNA group, the Mgat4a mRNA (\[0.53±0.2\] vs \[2.97±0.2\], \[2.90±0.1\]; P<0.05) and GnTⅣa (\[47.80±2.25\] vs \[39461±5.27), \[378.61±4.38\]) expression of the Mgat4a-shRNA group was significantly decreased, and the cell proliferation (inhibitory rate \[46.9±0.02\]% vs \[0.82±0.03\]%, 0; all P<0.01) and invasion (invasive cell numbers: \[18±3\] vs \[42±4\], \[38±3\], all P<0.05) capability were inhibited significantly. Conclusion: Down-regulation of lentivirus-mediated GnTⅣa expression inhibits the proliferation and invasion of mouse hepatoma Hca-F cells in vitro.
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Project Supported:
Project supported by the National Key Basic Research Development Program （973 Program） of China （No. 2012CB822103）, and the National Natural Science Foundation of China （No. 31000372，31170774）
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