Mobile website
Archive > Volume 20 Issue 6 > 2013,20(6):717-724. DOI:10.3872/j.issn.1007-385X.2013.06.015 Prev Next
Clinical Research
Expressions of growth arrest and DNA-damage-inducible 45 gene family members in esophageal squamous cell carcinoma and their clinical significance
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective : To investigate the expression of growth arrest and DNA-damage-inducible 45 (Gadd45) gene family members in esophageal squamous cell carcinoma (ESCC) and discuss its clinical significance. Methods: ESCC tissues and paracancerous tissues of one hundred and twenty-eight patients with ESCC from the Fourth Hospital Affiliated to Hebei Medical University during 2004-2008 were included in this study. The expressions of Gadd45A, Gadd45B and Gadd45G mRNA in the ESCC tissues and paracancerous tissues were detected by RT-PCR. The protein expressions of Gadd45A, Gadd45B and Gadd45G in ESCC tissues and paracancerous tissues were detected by using immunohistochemistry methods. The relation was analyzed between the expressions of Gadd45A, Gadd45B and Gadd45G and the clinical pathologic characteristics and prognosis of ESCC. Results: Compared to the paracancerous tissues, the expression of Gadd45A mRNA in the tumor tissues was significantly increased (\[0.8924±0.3457\] vs \[0.6123±0.2132\], P<005\], Gadd45B mRNA showed no significantly changes (\[0.8256±0.3110\] vs \[0.8173±0.3069), P>0.05\], Gadd45G mRNA was significantly decreased (\[0.3855±0.1210\] vs \[0.8214±0.3069\], P<0.05, and the expression of Gadd45G mRNA was closely associated with TNM stage and histological differentiation. The expression of Gadd45A in the paracancerous tissues was mainly stained in nucleolus, while part of the tumor tissues demonstrated a cytoplasm expression mode. Compared with the paracancerous tissues, the expression of Gadd45A in the tumor tissues was significantly increased (χ2=55.603, P=0.000), Gadd45B showed no significant difference (χ2=0.456, P=0.500), and Gadd45G was significantly decreased (χ2= 70.134, P=0.000). The expression of Gadd45G in patients in phase Ⅲ and Ⅳwas significantly lower than that in patients in phaseⅠ and Ⅱ (χ2=5.768, P=0.016), and was significantly lower in the low differentiation group than in the high and moderate differentiation groups (χ2=4.483, P=0.034). The 5-year survival rate of patients with Gadd45G-positive was significantly higher than that of patients with Gadd45G-negative (44% vs 21%, P<0.05); Cox model analysis shows that Gadd45G expression was an independent prognostic factor for ESCC. Conclusion: Aberrant expression of Gadd45A and Gadd45G may participate in the development and progression of ESCC.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81101854)
Clc Number:
