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MiR-29a inhibited costimulatory molecule B7-H3 expression and the invasion of glioma growth
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Abstract:
Objective : To determine the expression and possible roles of miR-29a and B7-H3 in glioma growth and invasion. Methods: Glioma tissue specimens were collected from 19 patients who underwent surgical glioma resection in the Department of Neurosurgery, Soochow University-Affiliated First Hospital between September, 2006 and December, 2010. Levels of miR-29a and B7-H3 mRNAs in the tissue specimens and human glioma U87 cells were determined by Real-time PCR. U87 cells were transfected with miR-29a mimics, and B7-H3 expression and invasive capacity in the transfectants were assessed by flow cytometry and transwell migration assay, respectively. The expression of invasion-related chemical chemokines was analyzed by flow cytometry, and the ability of miR-29a to bind to CXCR4 was predicted by the miRtarbase software. Results: In glioma tissue specimens, miR-29a and B7-H3 mRNA levels were inversely correlated. In vitro, miR-29a down-regulated B7-H3 mRNAs expression in U87 cells and miR-29a-mediated down-regulation of B7-H3 resulted in a significant decrease in the invasive ability but not proliferative activity in U87 cells. Parallel to down-regulation of B7-H3 expression, CXCR4 expression was also down-regulated in U87 cells transfected with miR-29a mimics. No miR-29a binding sites were detected in the CXCR4 gene. Conclusions: In human glioma, miR-29a can effectively down-regulate B7-H3 expression and inhibit invasive activity. It is likely that these effects of miR-29a were at least attributable dwon-regulated expression of CXCR4.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No. 30901313，No. 31100626，No.81301494）
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