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Liposome particles modified with transferrin and arginine-glycine-aspartic acid peptide: Laboratory preparation and its lung cancer cells targeting
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Abstract:
Objective: To optimize the preparation of liposome particles co-modified with transferrin and arginine-glycine-aspartic acid (RGD) peptide and evaluate the efficiency of the prepared particles in targeting lung cancer cells in vitro and in vivo ． Methods： Liposome particles modified, respectively, with vehicle (LP), transferrin (TF-LP), RGD peptide (RGD-LP) and TF and RGD (TF/RGD-LP) were prepared by a film-ultrasonic method. The appearance, size and Zeta potential of the particles were determined. The efficiency of these various particles in targeting lung cancer cells were assessed by cellular uptake assays in A549 cells and in a multicellular tumor spheroid model in vitro and by a imaging assay in solid tumors in nude mice in vivo . Results： TF/RGD-LP particles were 122.8±11.5 nm in diameter with an average Zeta potential of 6.4±3.85 mV. TF/RGF-LP particles were uptake by A549 cells 2.8, 2.2 and 3.9 times more efficiently than TF-LP, RGD-LP and LP particles respectively. In consistent with in vitro findings, TF/RGD-LP particles were most efficient among the types of particles tested in penetrating solid tumor spheroids in vivo. Conclusion： Co-modification with transferrin and RGD peptide can enhance the ability of liposomes to penetrate lung cancer cells and thus may offer a better antitumor agent delivery system.
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Project Supported:
Project supported by the Research Foundation of Education Bureau of Henan Province（No.1203068）
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