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Effects of Angelica sinensis polysaccharide on the biological characteristics and cytotoxicity-enhancing activity of CIK cells
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Abstract:
Objective: To characterize the biological features of Angelica sinensis polysaccharide (ASP) and evaluate its effect on the proliferation, immunotype and cytotoxicity of cytokine induced killer cells (CIK) derived from human peripheral blood mononuclear cells (PBMCs). Methods: PBMCs were obtained from healthy adult donors and induced to differentiate into CIK cells with interferon-γ (1 000 IU/ml) and anti-human CD3 (50 ng/ml). CIK cells were then treated with ASP every 3 days at 0, 12.5, 25, 50 and 100 μg/ml, respectively. At 10 days after treatment, the number of CIK cells was counted by computer-based cell counter, the proportion of CD3+CD4+, CD3+CD8+ and CD3+CD56+ cells were analyzed by flow cytometry , and the cytotoxicity of CIK cells were tested by CCK-8 method. Results: At the end of 16 day treatment, above 90% of CIK cells remained viable in all treatment groups, and the proliferative activity was significantly higher ( P <0.05) in CIK cells treated with 100 μg/ml ASP (56.98±3.00)×106 than in cells treated with 0 μg/ml ASP (43.81±2.14)×106. While the CD3+CD56+ cell ratio was significantly increased ( P <0.05) in CIK cells treated with 50 μg/ml ASP (26.65±3.71)% or 100 μg/ml ASP(28.36±4.28%) as compared with the control（20.75±3.56)% , there were no statistical differences in the proportion of CD3+CD4+ or CD3+CD8+ cells among the different groups ( P >0.05). The cytotoxic activity of CIK cells against K562 cells was significantly increased ( P <0.05) after treatment with 100 μg/ml ASP as compared with 0 μg/ml ASP at an effector-target ratio of 40∶1 (84.19±5.88)% vs （8.05±5.95)%, 20∶1（76.69±6.54)% vs (64.55±9.44)% or 10:1（72.32±9.22)% vs (61.45±8.22)%. Conclusion: Angelica sinensis polysaccharide can enhance the proliferative and cytotoxic activities of CIK cells in dose- and time-dependent manners.
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Project Supported:
Project support by the Major Science and Technology Projects in Gansu Province（No. 1102FKDA005）
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