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Effects of CXCR4 and CXCR7 downregulation by RNA interference on the biological behaviors of endometrial carcinoma HEC-1-A cells
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Abstract:
Objective: To investigate the effects of chemokine receptors CXCR4 and CXCR7 downregulation by siRNA on the biological characteristics of endometrial carcinoma cell HEC-1-A. Methods： siRNAs targeting CXCR4 and CXCR7 were transfected into HEC-1-A by using LipofectamineTM2000. The downregulation of CXCR4 and CXCR7 was assessed by RT-PCR and immunoblotting. The effects of altered CXCR4 and CXCR7 expression on cell proliferation, invasion and apoptosis were measured by CCK-8, Transwell and FCM respectively. Results： Both CXCR4 and CXCR7 were highly expressed in HEC-1-A cell lines. After transfected with specific siRNA, the expression of CXCR4 and CXCR7 gene and protein were downregulated markedly. The proliferation and invasion ability of HEC-1-A cells were significantly decreased compared to those transfected with control siRNA, and the cells were arrested in S phase. Conclusion： Knocking down CXCR4 and CXCR7 inhibits the proliferation and invasion ability of human endometrial cancer cell line HEC-1-A, suggesting that they are potential targets for endometrial cancer therapy.
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Project Supported:
Project supported by the Scientific Research Foundation of Shandong Province for Outstanding Young Scientist Award(No. BS2009SW002), and the Natural Science Foundation of Shandong Province(No. ZR2013HM012)
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