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Effect of annexinA7 knockdown on the proliferation and apoptosis of stomach carcinoma MGC-803 cells
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Abstract:
Objective: To investigate the effect of ANXA7 on the proliferation and apoptosis of human stomach carcinoma MGC-803 cells by downregulating ANXA7 expression using RNA interference technique. Methods: The MGC-803 cells were transfected with lipofectineTM2000 alone (blank control), scrambled siRNA (negative control), and siRNA targeted ANXA7 (experiment group). After 48 hours, ANXA7 expression was examined by immunoblotting. The proliferation and growth of transected cells were assessed by MTT and colony formation assays. Apoptosis of the cells was analyzed by flow cytometry. Results: The expression of ANXA7 was significantly decreased in cells transfected with ANXA7 siRNA compared with these in the two controls \[(21.79±1.72)% vs (99.87±5.13)% and (93.45±4.89)%, P <0.05\]. When ANXA7 expression was decreased, the proliferation and colony formation abilities of MGC-803 cells were markedly inhibited \[(0.97±0.06) vs (1.21±0.07) and (1.25±0.08), P <0.05; and (183±21) vs (363±35) and (348±27), P <0.05\], whereas the apoptosis of the cells was not affected statistically \[ P >0.05\]. Conclusion: ANXA7 regulates the proliferation and colony formation of MGC-803 cells, and appears not involved in their apoptosis.
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Project Supported:
Project supported by the Key Subjects in University of Hebei Province \[No.2013-4\]
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