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Expression of Neuritin in human non-small lung cancer-vascular endothelial cells
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Abstract:
Objective: To investigatethe expression of Neuritin in non-small lung cancer-vascular endothelial cells (NSCLC-VECs) and its potential role in the development ofvessels in non-small lung cancer. Methods: We have cultivated and identified NSCLC-VECs and human pulmonary microvascular endothelial cells (HPMEC) by using anti-FactorⅧ and CD34 antibodies. The expression ofneuritin in these cells were examined through RT-PCR and immunoblotting. Results: After pressurized filtration, we obtainedstably passaged NSCLC-VECs and HPMECs, which express endothelial cells specific markers FactorⅧ and CD34. The level of neuritin mRNA in NSCLC-VECs is significantly higher than that of HPMEC\[(0.95±0.09） vs （0.64±0.05）， P <0.05\]. At protein level, neuritin expression in NSCLC-VECs is more than 4-fold higher than that in HPMEC\[(1.15±0.11） vs （0.27±0.14）， P <0.05\]. Conclusion： Neuritin isoverexpressed in human NSCLC-VECs andmay contribute to tumor angiogenesis. The differentialexpression also suggests that neuritinis a potential therapeutictarget fornon-small cell lung cancer.
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Project Supported:
Project supported by Scientific Research Innovation Fund of Xinjiang Medical University（No. XJC201159）
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