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Expressions of ADAMTS1 and ADAMTS18 in esophageal squamous cell carcinoma and its clinical significance
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Abstract:
Objective: The present study was undertaken to examine the expression of ADAMTS1 and ADAMTS18 at mRNA and protein levels in esophagus squamous cell carcinoma (ESCC) for exploring their potential roles in the development and progress of the cancer. Methods: Tumor and adjacent noncancerous tissues were collected through surgical resection from 72 ESCC patients admitted to the Fourth Affiliated Hospital of Hebei Medical University between June 2008 and August 2011. The expression of ADAMTS1 and ADAMTS18 in ESCC tumor and adjacent tissues was examined with RT-PCR and immunohistochemistry to detect corresponding mRNA and protein. Results：①The level of ADAMTS1 mRNA in ESCC tumor tissues was significantly lower than that in adjacent non-tumor tissues (0.394±0.123 vs 0.895±0.276) ( P <0.01). Moreover, the level of ADAMTS1 mRNA in ESCC tumor tissues from patients with lymph node metastasis was significantly higher than that in ESCC tumor tissues from patients without lymph node metastasis (0.482±0.157 vs 0.298±0.102) ( P <0.05). ②The expression of ADAMTS18 mRNA in ESCC tumor tissues was significantly lower than that in adjacent non-tumor tissues (0.361±0.115 vs 0.879±0.265) ( P <0.01). Further, The mRNA level of ADAMTS18 mRNA in high/moderate differentiated ESCC was significantly higher than that in low differentiated ESCC (0.496±0.153 vs 0.232±0.088) ( P <0.05). ③The immunohistochemical positive rates of ADAMTS1 and ADAMTS18 in ESCC were significantly lower than that in adjacent non-tumor tissues \[38.9%(28/72) and 34.7%(25/72) vs 94.4%(68/72) and 93.1%(67/72) ( P <0.01)\]. Similar to its mRNA level, ADAMTS18 expression in high/moderate differentiated ESCC was significantly higher than that in low differentiated group \[45.4%(20/44) vs 17.8%(5/28) ( P <0.05)\]. ④There was no significant correlation between the expression of ADAMTS1 and ADAMTS18 in ESCC ( χ 2=1.338, P >0.05). Conclusion： Aberrant low expression of ADAMTS1 is associated with the development of ESCC and it lymph node metastasis, whereas reduced ADAMTS18 expression is closely related to ESCC development and its differentiation status. There are no correlation between the expression of ADAMTS1 and ADAMTS18 in ESCC.
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Project Supported:
Project supported by the Natural Science Foundation of Hebei Province (No. H2015206196)
Clc Number:
