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Diagnostic value of KISS1 mRNA detection in peripheral blood from patients with epithelial ovarian cancer
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Abstract:
Objective: To assess the diagnostic value of KISS1 mRNA in peripheral blood from patients with epithelial ovarian cancer (EOC) through comparison with CA125 measurement. Methods: Peripheral bloods were collected from 20 healthy individuals and 40 EOC patients, admitted into the Second Affiliated Hospital of Nantong University between January 2010 and January 2014, the day before the planned operations. The level of KISS1 mRNA in these blood samples was quantitated by RT-PCR, and in comparison, CA125 level in the same group of samples was measured by a chemiluminescence-based immunoassay. Results: KISS1 mRNA level in the peripheral blood from EOC patients of all stages was significantly higher than that of the healthy group ( P <0.01). However, the mRNA level in blood from patients with early EOC (FIGO Ⅰ & Ⅱ) was not significantly different from that of the advanced EOC (FIGO Ⅲ & Ⅳ) patients ( P >0.05). In contrast, CA125 level of the advanced EOC group was significantly higher than that of the early EOC group and the healthy group ( P <0.01). However, no significant differences of CA125 levels were found between the early EOC patients and the healthy volunteers ( P >0.05). When the cutoff values of receptive operator character curve (ROC) for the KISS1 mRNA were set to be 0.51 and 0.72, the corresponding positive predictive values (PPV) were 0.58 and 1.0, and the negative predictive values (NPV) were 0.92 and 0.7 respectively. When the cutoff values were 20 U/ml and 100U/ml for CA125 levels, the PPV were 0.72 and 1.0, and the NPV were 0.87 and 0.81, indicating that both KISS1 mRNA and CA125 levels have a moderate diagnostic efficiency ( P =0.34) for EOC. While the KISS1 mRNA level has a higher diagnostic value than that of CA125 for early stages of EOC ( P =0.018), CA125 measurement is more valuable than the KISS1 mRNA level in diagnosing advanced EOC ( P <0.018). Conclusion: KISS1 mRNA in peripheral blood is a potential novel biomarker. When deployed together with CA125 assay, it would likely improve the diagnosis of EOC, especially for the early stage cancer.
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Project Supported:
Project supported by the Social Enterprise Technology Innovation and Demonstration Project of Nantong City (No. HS2013039）
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