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Effect of Jinfukang decoction on prevention and treatment of transplanted lung cancer in immunosenescence mouse model
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Abstract:
Objective:To study the preventive and therapeutic effects of Jinfukang decoction on lung carcinoma in immunosenescence mouse induced by D-galactose. Methods:Using SPSS 18.0 software, 10 male C57BL/6J mice were randomly selected as control group and 83 male C57BL/6J mice as model group. In the model group, mice were subcutaneously injected with D-galactose (150 mg/kg/d) in scruff for 42 days to construct immunosenescence mouse model, normal saline and Jinfukang decoction were applied to intervene. After construction of the mouse model, 10 mice in the control group and 20 mice in the model group were selected to detect vitalities of serum superoxide dismutase (SOD) and contents of serum malondialdegyde (MDA),and then removed spleens and thymuses from the mice to calculate index of the organs; Flow cytometry was used to examine the expressions of immunosenscene related membrane molecules in T cells of the spleen and the thymuses. The rest 63 immunosenescence mice in the model group were subcutaneously injected with LL2-Luc-M38 lung cancer cells in the front of the left armpit to construct the model of Lewis lung cancer, and the mice were randomly divided into normal saline group, Jinfukang prevention group and Jinfukang prevention + treatment group. Among them, 30 mice (10 mice each group) were sacrificed at 14th day to measure the tumor mass, and the rest 33 mice were observed for time of tumor formation and survival time. Results: (1) Compared with the control group, the indexes of spleen and thymus in the normal saline group significantly decreased (P<0.05 or P<0.01); expression of CD3+CD45RA+, CD3+CD25+ and CD3+CD28+ in the spleen and the thymus significantly decreased (all P<0.01) while expression of CD3+CD196+ and CD4+CD25+ in the organs significantly increased (P<0.01); the vitalities of serum SOD remarkably decreased (P<0.001) while contents of serum MDA increased (P<0.001). After intervention of Jinfukang decoction, in the model group the spleen indexes increased significantly (P<0.01), expression of CD3+CD45RA+ and CD3+CD28+ increased (P<0.01 or P<0.05) and expression of CD3+CD196+ and CD4+CD25+ decreased (P<001) in the spleen, while expressioin of CD3+CD25+ increased (P<0.01), expression of CD3+CD196+ in the thymus decreased (P<0.05), vitalities of serum SOD increased (P<0.01) and contente of serum MDA decreased (P<0.01) compared with the normal saline group. (2) After the immunosenescence model mice were subcutaneously injected with lung cancer cells ,times of tumor formation and survival in the Jinfukang prevention group were significantly longer than those in the normal saline group (P<005), especially in the Jinfukang prevention + treatment group survival time was more significantly longer than that in the normal saline group (P<0.01); the tumor mass in the Jinfukang prevention + treatment group was smaller than those in the normal saline group (P<0.01) and the Jinfukang prevention group (P<005), but there was no significant difference between the latter two groups (P<0.05). Conclusion: The immunosenescence of mouse can be induced by D-galactose; Jifukang decoction can time-dependently delay the immunosenescence of mouse, by which Jifukang decoction can prevent the occurrence and development of lung cancer, and can prolong survival period of the mice bearing lung umor.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No. 81173224，No. 81373621），and the First Group of National Post-Doctoral Project on Traditional Chinese Medicine Inheritance（No. 135818）
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