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p[5HRE]AFPp-p53/PEI-Fe3O4 magnetic nanoparticle combined with magnetic fluid hyperthermia inhibit hepatoma cells
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Abstract:
Objective:To establish a combined therapy of p[5HRE]AFPp-p53/PEI-Fe3O4 magnetic nanoparticle and Magnetic Fluid Hyperthermia (MFH) to treat liver carcinoma and improve the safety and efficacy of the treatment. Methods:Sub-clone was used to construct hepatoma targeted gene p[5HRE]AFPp-p53, which was then confirmed by enzyme digestion and gel electrophoresis. The PEI-Fe3O4 magnetic nanoparticles were prepared by coprecipitation method, and its surface characteristics were examined by transmission electron microscope, particle size analyzer and Fourier transform infrared spectrometer etc. The proliferation of cell lines transfected with p[HRE]AFPp-p53/ PEI-Fe3O4, and the inhibition effect of targeted gene therapy combined with MFH on HepG2 cell proliferation were detected by MTT method. Results: p[5HRE]AFPp-p53/PEI-Fe3O4 magnetic nanoparticles were successfully constructed. Comparing with negative control group and nanoparticles control group, the proliferation activity of HepG2 cells ([0.592±0.041] vs [1.052±0031]，[1.012±0.021], P<0.01) and SMMC772 cells ([0.813±0.042] vs [1.073±0.032], [1.182±0.052], P<0.01) was significantly inhibited in p[5HRE]AFPp-p53/PEI-Fe3O4 mediated gene treatment group, however the proliferation of nonhepatoma cells (L929 and LOVO) was not significantly inhibited (P>0.05). Comparing with MFH group and gene therapy group, the inhibition rate of HegG2 cell proliferation was significantly increased in gene+MFH group (3522%, 42.92% vs 76.11%, P<0.01).Conclusion: p[5HRE]AFPp-p53/PEI-Fe3O4 magnetic nanoparticle has specific killing effect on liver carcinoma cells; it will have synergistic effect when combined with MFH; the combined therapy is an anti-tumor treatment with high selection and good therapeutic effect.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No. 81271636）
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