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Clinical Research
Expressions of Rb and P-Rb in breast cancer tissues and their significance in TGF-β induced epithelial-mesenchymal transition in breast cancer cells
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Abstract:
Objective:To investigate the expression of retinoblastoma (Rb) and phosphate retinoblastoma (P-Rb) in human breast cancer tissues and their significance during the process of epithelial-mesenchymal transition (EMT). Methods: 65 samples from patients with breast cancer that surgically removed in the Fourth Clinical Hospital of Hebei Medical University from Jan. to Aug. 2009 were retrospectively examined in this study. Immunohistochemistry was used to examine the expression of Rb, P-Rb, CDH1 and Vimentin in the breast cancer tissues of 65 cases. The correlation between their expression and clinicopathological parameters and prognosis of the case wih breast cancer was analyzed. Real-time PCR and Western blotting were used to examine the expression change of Rb and P-Rb in TGFβ-induced EMT. Results: Expression rates of Rb and P-Rb in the breast cancer tissues were 55.38% and 50.77%, respectively. Expression of P-Rb was positively correlated with tumor size, clinical stages and lymph node metastasis. Expression rates of CDH1 and Vimentin in the breast cancer were 76.92% and 43.08% respectively. Expression of CDH1 negatively correlates with tumor size, clinical stages and lymph node metastasis, while expression of Vimentin positively correlates with tumor size, clinical stages and lymph node metastasis. Both expressions of Rb and P-Rb positively correlates with expression of CDH1, but negatively correlates with expression of Vimentin. Overall 5 years survival rate of patients with P-Rb positive breast cancer was significantly lower than that of patients with P-Rb negative breast cancer; overall 5 years survival rate of patients with CDH1 positive breast cancer was significantly higher than that of patients with CDH1 negative breast cancer; overall 5 years survival rate of patients with Vimentin positive breast cancer was significantly lower than that of patients with Vimentin negative breast cancer. Expression of Rb was down regulated and that of P-Rb up regulated in TGFβ induced EMT of HEML cells.Conclusion: Inactivation of Rb with phosphorylation affected EMT and correlated with breast cancer metastasis.
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Project Supported:
Project supported by the Science and Technology Support Program of Hebei Province(No.14277732D)
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