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Archive > Volume 23 Issue 2 > 2016,23(2):168-174. DOI:10.3872/j.issn.1007-385X.2016.02.003 Prev Next
Prompt Report
Expression and function of Twist-1 gene in myeloid leukemia cells
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Abstract:
Objective:To investigate the expression of Twist-1 gene in patients with leukemia and tumor cell lines of hematopoietic system and to discuss the effect of its overexpression on proliferation and apoptosis of myeloid leukemic cells. Methods: Expressions of Twist-1 mRNA of hematopoietic tumor cell lines and PBMCs in patients with acute myeloid leukemia (AML), acute lymphoid leukemia (ALL) or chronic myeloid leukemia (CML) as well as in normal healthy human were detected by Real-time PCR. Lentiviral vector for overexperession of Twist-1 ( pCDH1-Twist-1) were constructed and transduced into myeloid leukemic K562, U937 and KG-1a cell lines. Effect of Twist-1 on proliferation, colony forming, cell cycle and apoptosis of the leukemic cells was evaluated by cell counting, colony forming, flow cytometry and Annexin V/PI assays. Results: Expressions of Twist-1 in patients with AML and CML were significantly higher than that in control group(all P<0.05), but there was no significant difference between all patients group and control group (P>005). The overexpression and interference analyses demonstrated that overexpression of Twist-1 in myeloid leukemic K562, U937 and KG-1a cell lines promoted proliferation and colony formation of the tumor cells, and inhibited apoptosis of the cells. Howevere, interfering expression of Twist-1 had the opposite effect. Conclusion: Twist-1 was overexpressed in myeloid leukemic K562, U937 and KG-1a cell lines and its overexpression could promote proliferation of the leukemia cells and inhibit their apoptosis.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No. 81270634, 81470278），the Youth Program of Applied Basic Research Foundation of Tianjin (No. 15JCQNJC10300) , and the Science-Technology Foundation for Young Scientists of Chinese Academy of Medical Sciences & Peking Union Medical College（No. 33320140066）
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