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Archive > Volume 23 Issue 3 > 2016,23(3):360-365. DOI:10.3872/j.issn.1007-385X.2016.03.011 Prev Next
Basic Research
Effect of sophoridine on proliferation, invasion and relative signaling pathways of neuroglioma U87 cell
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Abstract:
Objective:To explore inhibitory effects of sophoridine on DNA topoisomerase I (DNA TOP I), epidermal growth factor receptor-tyrosine kinase (EGFR-TK), matrix metalloproteinase 2 (MMP-2) and aminopeptidase N (APN), and the machanism for that growth and invasion of neuroglioma U87 cells were inhibited by the sophoridine. Methods: Sophoridine at different concentrations were added into brain neuroglioma U87 line cells. Inhibitory effect of the sophoridine on growths of the U87 cells and HEB human brain astrocytes was determined with MTT assay, activity of the sophoridine on apoptosis-associated protein caspase-3 in the U87 cells was detected by enzyme-linked turbidimetry assay, Transwell tests were used to determe effect of the sophoridine on invasion ability of the U87 cells. Expression of NF-κB in the U87 cells was examed by a non radioactive EMSA reagent kit.Results: Growth inhibitory rate of the neuroglioma U87 cells increased constantly with increase of sophoridine concentration (5, 10, 25, 50 and 100μmol/L), but growth rate of the HEB cells did not inhibited obviously (\[11.23±1.18\]% vs \[2.43±0.29\]%, \[22.48±3.21\]% vs \[3.65±042\]%, \[43.21±4.09\]% vs \[4.03±0.55\]%, \[57.31±5.09\]% vs \[5.21±0.43\]%, \[77.98±6.9\]% vs \[722±0.78\]%，all P<0.05). Compared with the blank control group, invasion ability of the U87 cells was significantly inhibited as presence of the sorphoridine (\[87.43±7.33\]%, \[65.12±6.16\]%, \[50.63±4.56\]%, \[35.32±4.04\]%, \[23.46±2.32\]% vs \[120.32±932\]%, all P<0.05). Half inhibitory rates (IC50) of the sorphoridin on DNA TOP I, EGFR-TPK, APN and MMP-2 were (\[22.43±2.21\], \[31.25±3.09\], \[6.32±0.32\] and \[8.23±0.63\] μmol/L) respectively. But activity of apoptosis protein caspase-3 in the U87 cell was enhanced and the sorphoridin down-regulated expression of NF-κB signaling. Conclution: The sorphoridin with low toxicity could reduce activities of DNA TOP I, EGFR-TPK, APN and MMP-2, down-regulate NF-κB signal pathway and activate apoptosis protein caspase-3, by which invasion and proliferation of the neuroglioma U87 cell and its signaling pathway could be inhibited.
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Project Supported:
Project supported by the Medical Science and Technology Research of Henan Province（No.2011020091）
Clc Number:
