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Archive > Volume 23 Issue 3 > 2016,23(3):366-370. DOI:10.3872/j.issn.1007-385X.2016.03.012 Prev Next
Basic Research
The effect of Yiqijianpi anticancer on expression of PKC, PKCδ and PKCε in mice colon cancer tissues
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Abstract:
Objective:To study the influence of Yiqijianpi anticancer method on the expression of PKC (protein kinase C) and its subtypes (PKCδ and PKCε) in transplanted colon cancer tissues. Methods: After the establishment of subcutaneous xenograft model (human colon cancer cell line HT-29), the mice were randomly divided into 3 groups: model group, Yiqijianpi group, Yiqijianpi anticancer group. The Chinese herbs used in Yiqijianpi group were composed of Radix pseudostellariae, Poria, Atractylodes, Licorice root, Pinellia and dried orange peel etc. In addition to these, the herbs used in replenishing and anticancer group had additional Iphigenia, Tuckahoe, Zhejiang fritillaria, and Hedyotis diffusa willd. After 14 days of administration, the protein and mRNA expressions of PKC, PKCδ and PKCε were detected by RT-PCR and Western blotting. Results: （1） The mRNA and protein expressions of PKC were lower in the xenograft tissues of Yiqijianpi anticancer group than those of the model group and Yiqijianpi group (\[0.412±0.040\] vs \[0.596±0021\], \[0.540±0.015\] and \[0.261±0.019\] vs \[0.665±0.016\], \[0.498±0.018\]，P<0.05 , P<0.01)；（2）the mRNA and protein expressions of PKCδ were higher than those of the model group and Yiqijianpi group （\[0.410±0030\] vs \[0.233±0025\], \[0.261±0.034\] and \[0.516±0.029\] vs \[0.301±0.041\], \[0.361±0.044\]，all P<0.01);（3）the mRNA and protein expressions of PKCε were lower than those of the model group and Yiqijianpi group (\[0.215±0021\] vs \[0.362±0.021\], \[0.314±0.031\] and \[0.224±0.029\] vs \[0.368±0.044\], \[0.359±0029)，both P<0.01\].Conclusion: Yiqijianpi anticancer method had an inhibitory effect on PKC, PKCε and a promotive effect on PKCδ in transplanted colon cancer tissues, and this may be one of the mechanisms that this anticancer method works on colon cancer.
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Project Supported:
Project supported by the Traditional Chinese Medicine Speciality Construction of Liaoning Province (No.2013-Lnzyxzk-01) and the Doctor Scientific Research Foundation of Liaoning Province（No.201501022）
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